Metal- and Affinity-Specific Dual Labeling of Cysteine-Rich Proteins for Identification of Metal-Binding Sites
نویسندگان
چکیده
منابع مشابه
Metal Binding Sites in Proteins
Metal ions play a critical role in living systems. About one third of proteins need to bind metal for their stability and/or function. In this review, current sequence based and structure based methods for metal binding site prediction will be presented, with emphasis on the CHED and SeqCHED methods of prediction from apo-protein structures and protein sequences having homologs (even remote) in...
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ساخت الکترودهاِی اصلاح شده ِیکِی از چالشهاِی همِیشگِی در دانش شیمِی بوِیژه شیمِی تجزیه مِی باشد ،که با در نظر گرفتن سادگِی ساخت، کاربردی بودن و ارزان بودن روش مِی توان به باارزش بودن چنِین سنسورهاِی پِی برد.آنچه که در ادامه آورده شده به ساخت و کاربرد الکترودهاِی اصلاح شده با استفاده از نانو ذرات در اندازه گِیرِی ولتامترِی آهن وکادمِیم اشاره دارد. کار اول اختصاص دارد به ساخت الکترود خمِیر کربن اصلاح شده با لِیگاند داِ...
15 صفحه اولEngineering metal-binding sites in proteins.
Metal-binding sites have been engineered into both de novo designed and naturally occurring proteins. Although the redesign of existing metal-binding sites in naturally occurring proteins still offers the most promise for a successful design, the more challenging goal of engineering metal-binding sites in de novo designed proteins and peptides is being achieved with increasing frequency. Creati...
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To further study the steroid binding site of 20&hydroxysteroid dehydrogenase (EC 1.1.1.53) by affinity labeling, 21[2’JH]bromoacetylaminoprogesterone was synthesized. The steroid inactivates the enzyme in a time-dependent and irreversible manner which follows pseudo-first order kinetics. Incorporation of 2 mol of 21-[2’-3Hlbromoacetylaminoprogesterone accompanies inactivation of 1 mol of enzyme...
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The metalloproteome is defined as the set of proteins that have metal-binding capacity by being metalloproteins or having metal-binding sites. A different metalloproteome may exist for each metal. Mass spectrometric characterization of metalloproteomes provides valuable information relating to cellular disposition of metals physiologically and in metal-associated diseases. We examined the Cu an...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2020
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.0c01604